Event Title
Regulation of Mouse Endothelial Cell Growth in Culture
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences, Cell and Molecular Biology
Mentor Information
David Kurjiaka, kurjiakd@gvsu.edu
Department
Biomedical Sciences
Location
Kirkhof Center KC14
Start Date
13-4-2011 2:00 PM
End Date
13-4-2011 3:00 PM
Keywords
Life Science
Abstract
Intercellular exchange of molecules between neighboring cells through gap junctions is involved in cellular growth regulation. In vascular endothelial cells, the gap junction protein Cx37 is only present in non-dividing cells. Cx43 replaces Cx37 when the endothelium is damaged and these cells must divide. As a first step in addressing gap junction's role in endothelial cell growth regulation, this study will determine whether the growth of cultured mouse endothelial cells (bEnd.3) can be arrested by serum deprivation: 10% FBS was replaced with 2% to 0% FBS. Contrary to expectations, bEnd.3 cells continued to grow in low FBS media. However, the high glucose media these endothelial cells were grown in stimulates the secretion of growth factors. When the media was changed regularly (every 24 or 48 hrs) to reduce endogenous growth factor concentrations, the growth of these cells was slowed (even in 10% FBS) indicating that growth factor release contributes to the growth of bEnd.3 cells.
Regulation of Mouse Endothelial Cell Growth in Culture
Kirkhof Center KC14
Intercellular exchange of molecules between neighboring cells through gap junctions is involved in cellular growth regulation. In vascular endothelial cells, the gap junction protein Cx37 is only present in non-dividing cells. Cx43 replaces Cx37 when the endothelium is damaged and these cells must divide. As a first step in addressing gap junction's role in endothelial cell growth regulation, this study will determine whether the growth of cultured mouse endothelial cells (bEnd.3) can be arrested by serum deprivation: 10% FBS was replaced with 2% to 0% FBS. Contrary to expectations, bEnd.3 cells continued to grow in low FBS media. However, the high glucose media these endothelial cells were grown in stimulates the secretion of growth factors. When the media was changed regularly (every 24 or 48 hrs) to reduce endogenous growth factor concentrations, the growth of these cells was slowed (even in 10% FBS) indicating that growth factor release contributes to the growth of bEnd.3 cells.