Event Title
Construction and Use of Viral Nato3 Overexpression Vector in the Developing Neural Tube of Gallus gallus.
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology
Mentor Information
Merritt Taylor, taylomer@gvsu.edu
Department
Biomedical Sciences
Location
Henry Hall Atrium 35
Start Date
13-4-2011 12:00 PM
End Date
13-4-2011 1:00 PM
Keywords
Health, Illness, and Healing, Life Science
Abstract
Nato3 is a basic helix-loop-helix gene endogenously expressed along the ventral midline of the developing central nervous system throughout gestation. Dopamine neurons and glia-like cells arise from the neural progenitors in the floor plate of the midbrain in midgestation, but little is known about the role of floor plate specific genes late in development. In order to determine the role of Nato3 in floor plate cell differentiation. We generated a retroviral vector containing Nato3 using a modified RCAS retroviral system. RCAS, derived from an avian retrovirus, can integrate into the genome of infected chick cells to allow persistent expression of Nato3 into late development. We used a modified form of RCAS, G-RCAS, which allows for the integration of Nato3 into the G-RCAS vector through a the Gateway system. We are currently generating retrovirus specific for infection of chick tissue using tissue culture and microinjection methods.
Construction and Use of Viral Nato3 Overexpression Vector in the Developing Neural Tube of Gallus gallus.
Henry Hall Atrium 35
Nato3 is a basic helix-loop-helix gene endogenously expressed along the ventral midline of the developing central nervous system throughout gestation. Dopamine neurons and glia-like cells arise from the neural progenitors in the floor plate of the midbrain in midgestation, but little is known about the role of floor plate specific genes late in development. In order to determine the role of Nato3 in floor plate cell differentiation. We generated a retroviral vector containing Nato3 using a modified RCAS retroviral system. RCAS, derived from an avian retrovirus, can integrate into the genome of infected chick cells to allow persistent expression of Nato3 into late development. We used a modified form of RCAS, G-RCAS, which allows for the integration of Nato3 into the G-RCAS vector through a the Gateway system. We are currently generating retrovirus specific for infection of chick tissue using tissue culture and microinjection methods.