Event Title
Analysis of a Cyclic Peptide Library to Identify Proteins Which Effect hilA Expression and Invasion in Salmonella
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
M. Aaron Baxter
Department
Biomedical Sciences
Location
Henry Hall Atrium 45
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
Salmonella Typhimurium is a gram-negative bacterium that produces a self-limiting, localized gastroenteritis in humans upon ingestion of contaminated food or water. Salmonella infection depends upon a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells of the small intestine. The expression of the SPI-1 genes is controlled via the sensing of various environmental conditions through multiple pathways. When conditions are optimal for invasion, these various environmental signals induce the SPI-1 transcriptional activator hilA. In collaboration with Brad Jones at the University of Iowa, we have identified some plasmids which produce cyclic peptides responsible for a reduction in expression of a hilA::lacZY reporter in E. coli and in Salmonella Typhimurium. The effects of these plasmids have also been quantified through in vivo invasion assays.
Analysis of a Cyclic Peptide Library to Identify Proteins Which Effect hilA Expression and Invasion in Salmonella
Henry Hall Atrium 45
Salmonella Typhimurium is a gram-negative bacterium that produces a self-limiting, localized gastroenteritis in humans upon ingestion of contaminated food or water. Salmonella infection depends upon a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells of the small intestine. The expression of the SPI-1 genes is controlled via the sensing of various environmental conditions through multiple pathways. When conditions are optimal for invasion, these various environmental signals induce the SPI-1 transcriptional activator hilA. In collaboration with Brad Jones at the University of Iowa, we have identified some plasmids which produce cyclic peptides responsible for a reduction in expression of a hilA::lacZY reporter in E. coli and in Salmonella Typhimurium. The effects of these plasmids have also been quantified through in vivo invasion assays.