Event Title
Tools for Monitoring the Effects of Gene Manipulation on Cell Differentiation in the Embryonic Chick Midbrain
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
Merritt Taylor
Department
Biomedical Sciences
Location
Kirkhof Center KC58
Start Date
11-4-2012 9:00 AM
Keywords
Information, Innovation, and Technology, Health, Physical Science
Abstract
The chick embryonic midbrain offers an effective model to monitor the effects of gene overexpression or knockdown on neural progenitor differentiation at multiple different points during development. To better characterize the effect of gene manipulation, we are analyzing the expression pattern of midbrain progenitor domains in the normal embryonic chick mesencephalon using markers that have been established in the mouse. We also have developed a quantitative PCR approach to detect changes in midbrain progenitor marker expression in response to experimental manipulation of gene expression using in ovo electroporation. These approaches will allow investigators to readily screen for changes in progenitor differentiation and be able to better analyze the effect of genes on the differentiation of mesecephalic progenitors.
Tools for Monitoring the Effects of Gene Manipulation on Cell Differentiation in the Embryonic Chick Midbrain
Kirkhof Center KC58
The chick embryonic midbrain offers an effective model to monitor the effects of gene overexpression or knockdown on neural progenitor differentiation at multiple different points during development. To better characterize the effect of gene manipulation, we are analyzing the expression pattern of midbrain progenitor domains in the normal embryonic chick mesencephalon using markers that have been established in the mouse. We also have developed a quantitative PCR approach to detect changes in midbrain progenitor marker expression in response to experimental manipulation of gene expression using in ovo electroporation. These approaches will allow investigators to readily screen for changes in progenitor differentiation and be able to better analyze the effect of genes on the differentiation of mesecephalic progenitors.