Event Title
Development of an Efficient Capillary Isoelectric Focusing Method for Separating Bacteria and Fungi Cells
Presentation Type
Poster/Portfolio
Presenter Major(s)
Chemistry
Mentor Information
Andrew Lantz
Department
Chemistry
Location
Henry Hall Atrium 45
Start Date
10-4-2013 1:00 PM
End Date
10-4-2013 2:00 PM
Keywords
Life Science, Physical Science
Abstract
Identifying and quantifying the bacteria, fungi, or viruses responsible for infectious contamination in food, biological samples, or water sources requires significant time and/or cost for analysis. A quick, efficient, and less expensive means for separating and analyzing complex biological samples for bacteria, fungi, and viruses would be highly beneficial. Capillary electrophoresis(CE)has the potential to be used for such analyses, including capillary isoelectric focusing (CIEF) which can separate analytes by their characteristic isoelectric point (pI). Here, we work to develop an efficient method using CIEF to separate microorganisms in biological samples. CIEF is capable of focusing cells and also measure their pI values by using a pI based calibration curve of molecular zwitterions. This research contributes to the development of a method that could replace current techniques such as growth plate methods and PCR.
Development of an Efficient Capillary Isoelectric Focusing Method for Separating Bacteria and Fungi Cells
Henry Hall Atrium 45
Identifying and quantifying the bacteria, fungi, or viruses responsible for infectious contamination in food, biological samples, or water sources requires significant time and/or cost for analysis. A quick, efficient, and less expensive means for separating and analyzing complex biological samples for bacteria, fungi, and viruses would be highly beneficial. Capillary electrophoresis(CE)has the potential to be used for such analyses, including capillary isoelectric focusing (CIEF) which can separate analytes by their characteristic isoelectric point (pI). Here, we work to develop an efficient method using CIEF to separate microorganisms in biological samples. CIEF is capable of focusing cells and also measure their pI values by using a pI based calibration curve of molecular zwitterions. This research contributes to the development of a method that could replace current techniques such as growth plate methods and PCR.