Faculty Scholarly Dissemination Grants


Homeorhetic Adaptation During the Pregnancy to Lactation Transition: Role of Solute Transporter Genes in Key Metabolic Tissues


Cell & Molecular Biology


College of Liberal Arts and Sciences

Date Range



Control of solute transport in the lactating mammary gland in tandem with adjustments in other key metabolic tissues, such as adipose and liver is pivotal, both in supporting milk synthesis and maintaining homeorhesis during lactation. Therefore, the goal of this study was to identify a more comprehensive cohort of solute transporter genes in mammary, liver, and adipose tissue that are transcriptionally controlled during the pregnancy to lactation transition. Total RNA was isolated from mammary, liver and adipose tissues collected from rat dams on day 20 of pregnancy (P20) and day 1 of lactation (L1) and gene expression was measured using Rat 230 2.0 Affymetrix GeneChips. Parametric gene set enrichment analysis method was utilized to identify transcriptional differences between P20 and L1 tissues. The foremost upregulated genes observed in the three tissues included amino-acid transporter, Slc1a2 (7.2 fold, FDR <0.001), nucleoside transporter, Slc28a3 (6.6 fold, FDR <0.001) and Zinc transporter, Slc30a5 (4.4 fold, FDR <0.001) in the mammary; sodium-dependent phosphate transporter, Slc34a2 (11.2 fold, FDR <0.001), sodium- and chloride-dependent GABA transporter, Slc6a1 (3.3 fold, FDR <0.001) and monocarboxylic acid transporter, Slc16a13 (2.1 fold, FDR <0.001) in the liver; sodium/chloride transporter, Slc12a3 (2.4 fold, FDR <0.01), facilitated glucose transporter, Slc2a9 (1.8 fold, FDR <0.05) and fatty acid transporter, Slc27a3 (1.6 fold, FDR <0.05) in the adipose tissue from P20 to L1. The leading downregulated transcripts observed in the three tissues included organic cation transporter, Slc22a17 (-4.3 fold, FDR <0.001), mitochondrial solute transporter, Slc22a17 (-3.4 fold, FDR <0.001) and facilitated glucose transporter, Slc2a8 (-3.0 fold, FDR <0.001) in the mammary; glucose/fructose transporter, Slc2a5 (-2.1 fold, FDR <0.1), sodium/chloride transporter, Slc12a3 (-1.8 fold, FDR <0.01), and mitochondrial transporter, Slc25a42 (-1.8 fold, FDR <0.1) in liver; glucose-6-phosphate transporter, Slc37a4 (-2.6 fold, FDR <0.01), facilitated glucose transporter, Slc2a4 (-2.5 fold, FDR <0.01) and amino acid transporter, Slc1a1 (-1.8 fold, FDR <0.05) in adipose tissue from late pregnancy to early lactation. Our results shows that among the strategies employed by the dam to support the rising demands of homeorhesis during pregnancy to lactation transition is careful orchestration of steady-state expression of solute transporters of various classes among multiple tissues.

Conference Name

44th Annual Meeting of the Society For Study of Reproduction

Conference Location

Portland, Oregon

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