Event Title

Exposure to Hypergravity During Pregnancy and Early Lactation Alters Abundance of Cytoskeletal and Extracellular Matrix Proteins in a Rat Model

Location

Exhibition Hall, DeVos Center

Start Date

10-4-2012 3:00 PM

Description

PURPOSE: Complete functional differentiation of the mammary gland is dependent on an integral cytoskeletal support structure and hormonal direction. Furthermore, prolactin released from the brain is indispensable for initiation and maintenance of lactation in all the species so far studied. However, intact cytoskeletal architecture is pivotal for prolactin-mediated development. Therefore, the objective of this study was to determine the effects of chronic hypergravity (HG) exposure from mid-pregnancy to early lactation on pre-partum/postpartum abundance of the cellular scaffolding and connective tissue proteins in the rat mammary gland. SUBJECTS: One group of pregnant Sprague Dawley rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). Another control (1g) and experimental (2g) groups were investigated from days 11 of pregnancy through days 1 (P1) and 3 (P3) postpartum. MATERIALS AND METHODS: On G20, P1 and P3, mammary tissue was collected and processed for immunohistochemical quantification of proteins associated with cellular scaffolding (actin, tubulin cytokeratin, vimentin). RESULTS: At G20, and P3 significant (pCONCLUSION: Our results suggest that abnormal cytoskeletal protein quantities correlate with the reduced mammary metabolic activity in HG-exposed rats.

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Apr 10th, 3:00 PM

Exposure to Hypergravity During Pregnancy and Early Lactation Alters Abundance of Cytoskeletal and Extracellular Matrix Proteins in a Rat Model

Exhibition Hall, DeVos Center

PURPOSE: Complete functional differentiation of the mammary gland is dependent on an integral cytoskeletal support structure and hormonal direction. Furthermore, prolactin released from the brain is indispensable for initiation and maintenance of lactation in all the species so far studied. However, intact cytoskeletal architecture is pivotal for prolactin-mediated development. Therefore, the objective of this study was to determine the effects of chronic hypergravity (HG) exposure from mid-pregnancy to early lactation on pre-partum/postpartum abundance of the cellular scaffolding and connective tissue proteins in the rat mammary gland. SUBJECTS: One group of pregnant Sprague Dawley rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). Another control (1g) and experimental (2g) groups were investigated from days 11 of pregnancy through days 1 (P1) and 3 (P3) postpartum. MATERIALS AND METHODS: On G20, P1 and P3, mammary tissue was collected and processed for immunohistochemical quantification of proteins associated with cellular scaffolding (actin, tubulin cytokeratin, vimentin). RESULTS: At G20, and P3 significant (pCONCLUSION: Our results suggest that abnormal cytoskeletal protein quantities correlate with the reduced mammary metabolic activity in HG-exposed rats.