Life Sciences | Molecular Biology


Dr. Martin Burg


The following is my annotation and transcription start site analysis report for contig70 in D. biarmipes to be submitted to the Genomics Education Partnership (GEP) at Washington University in St. Louis. The report was completed in accordance with the guidelines and template set forth by the GEP. There are eight complete genes found in contig70, for which I found the protein coding exon boundaries as well as the transcription start sites for all isoforms. All conclusions and reasoning for them are outlined in the report.

The GEP does genomics research, specifically comparing the genomes of all species of Drosophila. Students completing projects for the GEP analyze a portion of a genome of one of the species. The GEP collects all of this information and data and uses it for evolutionary analysis. Comparing the genomes of distinct species illuminates important regions of DNA, which are well conserved between species.

Students in CMB 440: Research Applications of Drosophila Genomics are expected to complete GEP reports for their individual projects. I took CMB 440 in Winter 2014, so this is the second project and report I have done. I decided to complete another report for my Honors Senior Project to assist Dr. Martin Burg in advancing the scope of the course. Before this semester, students were not expected to complete transcription start site (TSS) analysis for their projects. To ensure a smoother integration of TSS analysis, Dr. Burg asked me to become well versed in the process so I could teach it to students. I studied GEP materials and instructions over the course of the semester to compile a comprehensive instructional presentation on TSS analysis to give to the class. Along with the presentation, I made myself available once a week in class for students to ask me questions concerning TSS analysis in their individual projects.