Event Title
Impact of Hypergravity Exposure on Pregnant Rat Mammary Lobular Dimensions
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology
Mentor Information
Osman Patel, patelo@gvsu.edu
Department
Cell and Molecular Biology
Location
Kirkhof Center KC40
Start Date
13-4-2011 9:00 AM
End Date
13-4-2011 10:00 AM
Keywords
Environment, Health and Wellness, Life Science, Technology
Abstract
Mammary gland output is determined by the number of secretory epithelial cells. Moreover, growth and differentiation of the mammary gland predominately occurs during pregnancy. Previous studies show that exposure to hypergravity (HG) during pregnancy impacts metabolic activity of the dam during lactation. Therefore, the objective of this study is to evaluate the effects of hypergravity exposure from mid- to late pregnancy on pre-partum dimensions of the mammary gland lobules. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and processed for immunohistochemistry.Tissue was formalin fixed and paraffin embedded for histological and immunohistochemical characterization of cellular structures using antibody directed staining and image analysis of structure, area and component analysis.Immunolocalization studies of HG exposed and control rats are completed and quantification data will be presented.
Impact of Hypergravity Exposure on Pregnant Rat Mammary Lobular Dimensions
Kirkhof Center KC40
Mammary gland output is determined by the number of secretory epithelial cells. Moreover, growth and differentiation of the mammary gland predominately occurs during pregnancy. Previous studies show that exposure to hypergravity (HG) during pregnancy impacts metabolic activity of the dam during lactation. Therefore, the objective of this study is to evaluate the effects of hypergravity exposure from mid- to late pregnancy on pre-partum dimensions of the mammary gland lobules. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and processed for immunohistochemistry.Tissue was formalin fixed and paraffin embedded for histological and immunohistochemical characterization of cellular structures using antibody directed staining and image analysis of structure, area and component analysis.Immunolocalization studies of HG exposed and control rats are completed and quantification data will be presented.