Event Title
Performance of Control Experiments for Hyperbaric Therapy Research
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
Francis Sylvester
Department
Biomedical Sciences
Location
Kirkhof Center KC27
Start Date
10-4-2013 12:00 PM
End Date
10-4-2013 1:00 PM
Keywords
Health, Life Science
Abstract
Hyperbaric oxygen therapy increases the partial pressure of oxygen in vivo, allowing body tissues to absorb more oxygen. This continuation of an ongoing research project seeks to perform time control experiments to measure the effect of prolonged incubation under normal atmospheric conditions on vascular reactivity. Specifically, porcine pulmonary arteries will be dissected, incubated for 2 hours under normal atmospheric conditions, mounted in isolated organ baths, and responses to potassium chloride, phenylephrine, and nitroprusside will be recorded. Responses consist of changes in tension as generated by the smooth muscle in the arterial wall. We hypothesize that increased ATP production plays a role in the altered vascular reactivity observed in blood vessels following hyperbaric oxygen therapy. An additional control will be to conduct dose response experiments to exogenous ATP. This will lead to a greater understanding of the effects of hyperbaric therapy on the vascular system.
Performance of Control Experiments for Hyperbaric Therapy Research
Kirkhof Center KC27
Hyperbaric oxygen therapy increases the partial pressure of oxygen in vivo, allowing body tissues to absorb more oxygen. This continuation of an ongoing research project seeks to perform time control experiments to measure the effect of prolonged incubation under normal atmospheric conditions on vascular reactivity. Specifically, porcine pulmonary arteries will be dissected, incubated for 2 hours under normal atmospheric conditions, mounted in isolated organ baths, and responses to potassium chloride, phenylephrine, and nitroprusside will be recorded. Responses consist of changes in tension as generated by the smooth muscle in the arterial wall. We hypothesize that increased ATP production plays a role in the altered vascular reactivity observed in blood vessels following hyperbaric oxygen therapy. An additional control will be to conduct dose response experiments to exogenous ATP. This will lead to a greater understanding of the effects of hyperbaric therapy on the vascular system.