Article Title
Identification of Histidine Decarboxylase Expression using a Histamine Antibody Staining Technique
Abstract
Cells containing histamine were identified in a third instar brain of a normal Drosophila melanogaster larva using a whole-mount staining technique that detects histamine. The histamine antibody staining was performed on intact tissue to easily identify cells that likely use histamine as a neurotransmitter in the brain. Results indicate that mutants in the Hdc gene do not have detectable levels of histamine in the central brain of larvae. The methodology used and these results should allow an examination of mutants that may be defective in spatial regulation of the transmitter, histamine.
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