Event Title
Hyperbaric Oxygen Therapy Alters Vascular Reactivity Independent of ATP
Location
Hager-Lubbers Exhibition Hall
Description
PURPOSE: The purpose of this study was to record the acute effects of hyperbaric oxygen (HBO) on vascular reactivity and determine the potential role of ATP in mediating these effects. METHODS AND MATERIALS: Porcine pulmonary arteries were dissected and mounted in isolated organ baths to record changes in tension in response to potassium chloride (KCl, 15-60 mM), phenylephrine (Phe, 10-7-10-4 M), and sodium nitroprusside (SNP, 10-7-10-4 M) following a 2-hour exposure to HBO (1.75 atm). RESULTS: HBO augmented responses to KCl and Phe compared to control arteries exposed to room air or nitrogen at 1.75 atm as well as to room air at 1 atm. Earlier studies in our lab demonstrated that HBO similarly altered vascular reactivity in mesenteric arteries. We hypothesized that HBO increased ATP production in vascular smooth muscle leading to enhanced vascular reactivity. Consequently, ATP levels were measured in mesenteric arteries but no significant differences in ATP levels were observed regardless of hyperbaric treatment. CONCLUSION: These results suggest that HBO alters vascular reactivity independent of ATP.
Hyperbaric Oxygen Therapy Alters Vascular Reactivity Independent of ATP
Hager-Lubbers Exhibition Hall
PURPOSE: The purpose of this study was to record the acute effects of hyperbaric oxygen (HBO) on vascular reactivity and determine the potential role of ATP in mediating these effects. METHODS AND MATERIALS: Porcine pulmonary arteries were dissected and mounted in isolated organ baths to record changes in tension in response to potassium chloride (KCl, 15-60 mM), phenylephrine (Phe, 10-7-10-4 M), and sodium nitroprusside (SNP, 10-7-10-4 M) following a 2-hour exposure to HBO (1.75 atm). RESULTS: HBO augmented responses to KCl and Phe compared to control arteries exposed to room air or nitrogen at 1.75 atm as well as to room air at 1 atm. Earlier studies in our lab demonstrated that HBO similarly altered vascular reactivity in mesenteric arteries. We hypothesized that HBO increased ATP production in vascular smooth muscle leading to enhanced vascular reactivity. Consequently, ATP levels were measured in mesenteric arteries but no significant differences in ATP levels were observed regardless of hyperbaric treatment. CONCLUSION: These results suggest that HBO alters vascular reactivity independent of ATP.