Automating Elisa With Andrew+: Maximizing Efficiency In Assay Workflow
Location
Hager-Lubbers Exhibition Hall
Description
PURPOSE: The enzyme-linked immunosorbent assay (ELISA) is essential for detecting specific proteins or antibodies. This study evaluated the efficiency and accuracy of an automated ELISA protocol using the Andrew+ Pipetting Robot compared to the traditional manual approach for detecting Porcine Circovirus Type 2 (PCV2) antibodies. CHALLENGE: Manual ELISA procedures are time-consuming and prone to human errors, affecting consistency and throughput. The challenge was to assess whether automation could enhance accuracy, reduce errors, and improve workflow efficiency. EXPERIENCE: The study involved running 176 porcine serum samples in duplicate and triplicate using both manual and automated ELISA methods. The Andrew+ robot, programmed via OneLab software, automated sample dilution and plate preparation, whereas the manual method required hand pipetting. OUTCOME: The automated method demonstrated a 98.3% concordance rate with manual results while reducing hands-on time by 30 minutes per assay. At the Zoetis Kalamazoo facility alone, this translates to a potential annual time savings of 2,280 minutes, increasing productivity and standardization in PCV2 antibody detection. IMPACT: Automating ELISA assays with the Andrew+ system significantly improves efficiency, reproducibility, and data integrity. This advancement benefits both research and diagnostic workflows, setting the stage for broader applications in biological assay automation.
Automating Elisa With Andrew+: Maximizing Efficiency In Assay Workflow
Hager-Lubbers Exhibition Hall
PURPOSE: The enzyme-linked immunosorbent assay (ELISA) is essential for detecting specific proteins or antibodies. This study evaluated the efficiency and accuracy of an automated ELISA protocol using the Andrew+ Pipetting Robot compared to the traditional manual approach for detecting Porcine Circovirus Type 2 (PCV2) antibodies. CHALLENGE: Manual ELISA procedures are time-consuming and prone to human errors, affecting consistency and throughput. The challenge was to assess whether automation could enhance accuracy, reduce errors, and improve workflow efficiency. EXPERIENCE: The study involved running 176 porcine serum samples in duplicate and triplicate using both manual and automated ELISA methods. The Andrew+ robot, programmed via OneLab software, automated sample dilution and plate preparation, whereas the manual method required hand pipetting. OUTCOME: The automated method demonstrated a 98.3% concordance rate with manual results while reducing hands-on time by 30 minutes per assay. At the Zoetis Kalamazoo facility alone, this translates to a potential annual time savings of 2,280 minutes, increasing productivity and standardization in PCV2 antibody detection. IMPACT: Automating ELISA assays with the Andrew+ system significantly improves efficiency, reproducibility, and data integrity. This advancement benefits both research and diagnostic workflows, setting the stage for broader applications in biological assay automation.