Vitamin C, Histamine, Coronary Arteries


Chemicals and Drugs


The purpose of the study was to determine whether the vasoresponses of cross-sections of porcine left anterior descending arteries(LADs) would be adversely affected by being subjected to 3 hour incubation in a combination bath of histamine and vitamin C as compared to the controls. For each of the eight times the experiment was performed crosssection rings were cut from the same LAD then were mounted in isolated baths. Responses to vasoactive agents causing tension changes were recorded in grams by the force transducers the rings were mounted on. Each ring was incubated for 3 hours in physiologic saline solution(PSS) / Krebs Buffer, ascorbic acid(vitamin C) in PSS, histamine in PSS or a combination of vitamin C and histamine in PSS. Each ring’s response to vasoactive agents was again recorded. The difference in response to KCl was 2.54±1.62g in PSS only group, -0.418±1.80g in vitamin C group, -0.107±1.51g in the histamine group, -2.62±1.94g in the combination bath group. The loss in response to SNP was 0.856±.957g in PSS group, 2.084±.622g in the vitamin C group, 2.068±.591g in the histamine group, 4.711±1.01g in the combination bath group. None of the results for change in KCl response were statistically significant between any of the controls and the combination bath. This data suggests vasoconstriction of coronary arteries induced by KCl is not affected by incubation in a combination of vitamin C and histamine. Results for loss in response to SNP between PSS control and combination bath were statistically significant (p=0.023). This data shows arteries incubated in a combination of histamine and vitamin C have a reduced vasodilation response to SNP compared to those incubated in PSS. The results between vitamin C and histamine controls and the combination bath were not statistically significant (p=0.090). These results indicate a possible trend in the change of vasodilation response between combination bath and histamine and vitamin C controls. P-values were obtained by 1-way ANOVA using Student-Newman- Keuls post hoc analysis.