Date Approved
1-6-2022
Graduate Degree Type
Thesis
Degree Name
Biomedical Sciences (M.H.S.)
Degree Program
Biomedical Sciences
First Advisor
Eric Ramsson
Academic Year
2021/2022
Abstract
Stroke is one of the most detrimental neurological diseases worldwide. Duration, location, cause, and severity of stroke impact outcome and chance of survival. The only current therapeutic approach for acute ischemic stroke is thrombolysis; intense efforts aim to further understand the pathophysiology and develop effective treatments and therapies for stroke. In the last five years, there has been push to understand the anti-inflammatory, antioxidant, and neuroprotective effects of cannabidiol (CBD). CBD has the ability to regulate neuronal homeostasis and survival, doing so by preserving, rescuing, repairing and even replacing neurons after events that can cause potential damage. These properties are facilitated by targets of action such as the CB1 and CB2 receptors located in neurons and glial cells. The neuroprotective potential of CBD is promising for acute neurotraumatic events, so the aim of this study was to examine neuroprotective effects of CBD during stroke. Since cerebral ischemia, whether mild or severe, alters release and uptake of dopamine (DA) in the striatum, fast-scan cyclic voltammetry (FSCV) will be used to measure evoked DA after exposure to 3 different CBD concentrations (10 μM, 50 μM and 100 μM) and oxygen-glucose deprivation. The hypothesis is that pretreatment with CBD prior to stroke will demonstrate a neuroprotective effect. It’s expected to see higher evoked DA levels during stroke and better recovery rates. Results show CBD has an effect but is influenced by sex of the animal. DA levels didn’t drop as much in males exposed to 10 μM CBD during stroke suggesting a neuroprotective effect. There is a clear sex difference, but the cause is unknown. Further experimentation is required to fully understand why these sex differences arise.
ScholarWorks Citation
Floyd, Analis, "Neuroprotective Effect of Cannabidiol During an Ischemic Event" (2022). Masters Theses. 1032.
https://scholarworks.gvsu.edu/theses/1032
