Enhancement of fast scan cyclic voltammetry detection of dopamine with tryptophanmodified electrodes
Keywords
Electrode potentials, Oxidation, Voltammetry, Carbon fiber, Electron transfer, Amines, Microelectrodes, Serotonin
Disciplines
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Abstract
Fast scan cyclic voltammetry (FSCV) allows for real -time analysis of phasic neurotransmitter levels. Tryptophan (TRP) is an aromatic amino acid responsible for facilitating electron transfer kinetics in oxidoreductase enzymes. Previous work with TRP-modified electrodes showed increased sensitivity for cyclic voltammetry detection of dopamine (DA) when used with slower scan rates (0.05 V/s). Here, we outline an in vitro proof of concept for TRP-modified electrodes in FSCV detection of DA, and decreased sensitivity for ascorbic acid (AA). TRP-modified electrodes had a limit of detection (LOD) for DA of 2.480 ± 0.343 nM compared to 8.348 ± 0.405 nM for an uncoated electrode. Selectivity for DA/ascorbic acid (AA) was 1.107 ± 0.3643 for uncoated and 15.57 ± 4.184 for TRP-modified electrodes. Additionally, these TRP-modified electrodes demonstrated reproducibility when exposed to extended cycling. TRP-modified electrodes will provide an effective modification to increase sensitivity for DA.
Original Citation
Davis, S. E., Korich, A. L., & Ramsson, E. S. (2020). Enhancement of fast scan cyclic voltammetry detection of dopamine with tryptophan-modified electrodes. PLoS ONE, 15(7), 1–12. https://doi.org/10.1371/journal.pone.0235407
ScholarWorks Citation
Davis, Sarah E.; Korich, Andrew L.; and Ramsson, Eric S., "Enhancement of fast scan cyclic voltammetry detection of dopamine with tryptophanmodified electrodes" (2020). Peer Reviewed Articles. 11.
https://scholarworks.gvsu.edu/bms_articles/11
