Serotonin and Histamine Localization in the CNS of Drosophila melanogaster

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences

Mentor Information

Martin Burg, burgm@gvsu.edu

Department

Biomedical Sciences

Location

Kirkhof Center KC5

Start Date

13-4-2011 1:00 PM

End Date

13-4-2011 2:00 PM

Keywords

Life Science

Abstract

Histamine is present in cells of the Drosophila central nervous system (CNS), although the function of these cells has not been well established. The enzyme that catalyzes the formation of histamine is histidine decarboxylase (Hdc). Transgenic flies containing a gene fusion between the 5' transcriptional control region of the Hdc gene (pHdc) and the Green Fluorescent Protein (eGFP) have been shown to contain neurons that contained both histamine and the eGFP protein. This allows histaminergic cell visualization without the use of a histamine antibody, which is difficult to use in conjunction with other antibodies. The spatial relationship between histaminergic cells and cells containing another neurotransmitter, serotonin, was performed using immunocytochemical techniques with pHdc-eGFP larva. Results obtained suggest that the locations of histaminergic and serotonergic cells differ from each other, with more serotonergic cells being present in each segment of the CNS.

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Apr 13th, 1:00 PM Apr 13th, 2:00 PM

Serotonin and Histamine Localization in the CNS of Drosophila melanogaster

Kirkhof Center KC5

Histamine is present in cells of the Drosophila central nervous system (CNS), although the function of these cells has not been well established. The enzyme that catalyzes the formation of histamine is histidine decarboxylase (Hdc). Transgenic flies containing a gene fusion between the 5' transcriptional control region of the Hdc gene (pHdc) and the Green Fluorescent Protein (eGFP) have been shown to contain neurons that contained both histamine and the eGFP protein. This allows histaminergic cell visualization without the use of a histamine antibody, which is difficult to use in conjunction with other antibodies. The spatial relationship between histaminergic cells and cells containing another neurotransmitter, serotonin, was performed using immunocytochemical techniques with pHdc-eGFP larva. Results obtained suggest that the locations of histaminergic and serotonergic cells differ from each other, with more serotonergic cells being present in each segment of the CNS.