Event Title
The Effect of Nato3 Misexpression on Neural Progenitor Cell Differentiation in the Rostral Neural Tube.
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology, Biomedical Sciences
Mentor Information
Merritt Taylor, taylomer@gvsu.edu
Department
Biomedical Sciences
Location
Henry Hall Atrium 47
Start Date
13-4-2011 9:00 AM
End Date
13-4-2011 10:00 AM
Abstract
Nato3, a basic helix-loop-helix protein, is expressed in the floor plate region of the midbrain in the developing embryo. To determine if Nato3 is sufficient to induce floor plate cell characteristics in other regions of the developing neural tube, we have misexpessed Nato3 using in ovo electroporation. We are monitoring neural progenitors and their progeny that misexpress Nato3 during development using a bicistronic EGFP reporter expression vector. Using immunohistochemistry we have observed the effect of Nato3 misexpression on neural progenitors in the hindbrain and midbrain. In the hindbrain, Nato3 misexpression is not sufficient to induce DA neurogenesis, but could induce expression of some floor plate markers. Our data indicates that misexpression of Nato3 is sufficient to induce immature DA neuron markers in the posterior midbrain.
The Effect of Nato3 Misexpression on Neural Progenitor Cell Differentiation in the Rostral Neural Tube.
Henry Hall Atrium 47
Nato3, a basic helix-loop-helix protein, is expressed in the floor plate region of the midbrain in the developing embryo. To determine if Nato3 is sufficient to induce floor plate cell characteristics in other regions of the developing neural tube, we have misexpessed Nato3 using in ovo electroporation. We are monitoring neural progenitors and their progeny that misexpress Nato3 during development using a bicistronic EGFP reporter expression vector. Using immunohistochemistry we have observed the effect of Nato3 misexpression on neural progenitors in the hindbrain and midbrain. In the hindbrain, Nato3 misexpression is not sufficient to induce DA neurogenesis, but could induce expression of some floor plate markers. Our data indicates that misexpression of Nato3 is sufficient to induce immature DA neuron markers in the posterior midbrain.