Event Title
Analysis of a Cyclic Peptide Library to Identify Proteins That Effect hilA and fimZ Expression in Salmonella Invasion
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
M. Aaron Baxter, baxteraa@gvsu.edu
Department
Biomedical Sciences
Location
Henry Hall Atrium 93
Start Date
13-4-2011 1:00 PM
End Date
13-4-2011 2:00 PM
Keywords
Life Science
Abstract
Salmonella enterica serovar Typhimurium is a gram-negative bacterium that produces a localized gastroenteritidis upon ingestion of contaminated food or water. Pathogenesis demands Salmonella recognition of apt environmental conditions which activate a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells. The expression of the SPI-1 genes is controlled via the sensing of various environment conditions through multiple pathways. The central regulator of SPI-1 is the transcriptional activator hilA. Currently we have isolated 5 plasmids, producing cyclic peptides, which have shown to cause a reduction in hilA expression in E. coli. 3 out of the 5 plasmids have been quantified in Salmonella and negatively regulate hilA expression. The effects of the plasmids on hilA expression is currently being further analyzed through in vivo invasion assays.
Analysis of a Cyclic Peptide Library to Identify Proteins That Effect hilA and fimZ Expression in Salmonella Invasion
Henry Hall Atrium 93
Salmonella enterica serovar Typhimurium is a gram-negative bacterium that produces a localized gastroenteritidis upon ingestion of contaminated food or water. Pathogenesis demands Salmonella recognition of apt environmental conditions which activate a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells. The expression of the SPI-1 genes is controlled via the sensing of various environment conditions through multiple pathways. The central regulator of SPI-1 is the transcriptional activator hilA. Currently we have isolated 5 plasmids, producing cyclic peptides, which have shown to cause a reduction in hilA expression in E. coli. 3 out of the 5 plasmids have been quantified in Salmonella and negatively regulate hilA expression. The effects of the plasmids on hilA expression is currently being further analyzed through in vivo invasion assays.