Analysis of a Cyclic Peptide Library to Identify Proteins That Effect hilA and fimZ Expression in Salmonella Invasion

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences

Mentor Information

M. Aaron Baxter, baxteraa@gvsu.edu

Department

Biomedical Sciences

Location

Henry Hall Atrium 93

Start Date

13-4-2011 1:00 PM

End Date

13-4-2011 2:00 PM

Keywords

Life Science

Abstract

Salmonella enterica serovar Typhimurium is a gram-negative bacterium that produces a localized gastroenteritidis upon ingestion of contaminated food or water. Pathogenesis demands Salmonella recognition of apt environmental conditions which activate a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells. The expression of the SPI-1 genes is controlled via the sensing of various environment conditions through multiple pathways. The central regulator of SPI-1 is the transcriptional activator hilA. Currently we have isolated 5 plasmids, producing cyclic peptides, which have shown to cause a reduction in hilA expression in E. coli. 3 out of the 5 plasmids have been quantified in Salmonella and negatively regulate hilA expression. The effects of the plasmids on hilA expression is currently being further analyzed through in vivo invasion assays.

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Apr 13th, 1:00 PM Apr 13th, 2:00 PM

Analysis of a Cyclic Peptide Library to Identify Proteins That Effect hilA and fimZ Expression in Salmonella Invasion

Henry Hall Atrium 93

Salmonella enterica serovar Typhimurium is a gram-negative bacterium that produces a localized gastroenteritidis upon ingestion of contaminated food or water. Pathogenesis demands Salmonella recognition of apt environmental conditions which activate a 40 kb region of DNA known as Salmonella Pathogenicity Island 1 (SPI-1). The expressed SPI-1 proteins manipulate normal host cell function and direct the uptake of the bacteria into targeted cells. The expression of the SPI-1 genes is controlled via the sensing of various environment conditions through multiple pathways. The central regulator of SPI-1 is the transcriptional activator hilA. Currently we have isolated 5 plasmids, producing cyclic peptides, which have shown to cause a reduction in hilA expression in E. coli. 3 out of the 5 plasmids have been quantified in Salmonella and negatively regulate hilA expression. The effects of the plasmids on hilA expression is currently being further analyzed through in vivo invasion assays.