Event Title
The Use of High Resolution micro Ultrasound to Measure Hind Limb Perfusion in an Ischemia Induced Mouse Model
Presentation Type
Oral and/or Visual Presentation
Presenter Major(s)
Biomedical Sciences
Mentor Information
Brian Kipp
Department
Biomedical Sciences
Location
Kirkhof Center 2266
Start Date
11-4-2012 10:30 AM
Keywords
Information, Innovation, and Technology, Health, Technology
Abstract
High-Resolution micro Ultrasound is a valuable area of small animal imaging for use in pre-clinical research. This study utilizes a mouse model of Peripheral Arterial Disease (PAD), through induced ischemia of the hind limb by femoral artery/vein ligation. A method was developed using Power Doppler micro ultrasound to accurately and precisely measure hind limb blood flow over 5 weeks time. Monitoring the hind limb perfusion can confirm ligation for the PAD model showing up to 60 % decreased perfusion. Also, the scans allow evaluation of the pattern of reperfusion through quantitative data and qualitative 3D images. The tissue reperfusion data is used with immunohistochemistry to study the underlying biochemical pathways activated in micro-vessel regeneration, or angiogenesis. The use of micro ultrasound proves to be a valuable tool in gaining access to the hemodynamic properties of this mouse model as it is affected by an ischemic injury and as it adapts through angiogenesis.
The Use of High Resolution micro Ultrasound to Measure Hind Limb Perfusion in an Ischemia Induced Mouse Model
Kirkhof Center 2266
High-Resolution micro Ultrasound is a valuable area of small animal imaging for use in pre-clinical research. This study utilizes a mouse model of Peripheral Arterial Disease (PAD), through induced ischemia of the hind limb by femoral artery/vein ligation. A method was developed using Power Doppler micro ultrasound to accurately and precisely measure hind limb blood flow over 5 weeks time. Monitoring the hind limb perfusion can confirm ligation for the PAD model showing up to 60 % decreased perfusion. Also, the scans allow evaluation of the pattern of reperfusion through quantitative data and qualitative 3D images. The tissue reperfusion data is used with immunohistochemistry to study the underlying biochemical pathways activated in micro-vessel regeneration, or angiogenesis. The use of micro ultrasound proves to be a valuable tool in gaining access to the hemodynamic properties of this mouse model as it is affected by an ischemic injury and as it adapts through angiogenesis.