Required Modifications for the Expression and Purification of the E290K Mutant of Horse Heart Cytochrome C Peroxidase.

Presentation Type

Poster/Portfolio

Presenter Major(s)

Chemistry

Mentor Information

Cory DiCarlo

Department

Chemistry

Location

Henry Hall Atrium 89

Start Date

11-4-2012 9:00 AM

Keywords

Physical Science

Abstract

Expression of mutant horse heart cytochrome c peroxidase (HH CcP) was carried out using a recombinant bacterial expression system. Crystallization of HH CcP mutant E290K was realized with the formation of purified protein crystals. Although crystallization of this mutant was attained other mutant strains were found to be less receptive of the techniques used during the early stages of isolation. Crystal size was found to be diminutive and recrystallization for the sample will need to be performed to increase crystal dimensions for crystallography to be performed. Further development and honing of the procedures for cell cultures, protein isolation and purification was carried out through acclimatizing prior procedures to attain improved realizations of product though each stage of product collection.

This document is currently not available here.

Share

COinS
 
Apr 11th, 9:00 AM

Required Modifications for the Expression and Purification of the E290K Mutant of Horse Heart Cytochrome C Peroxidase.

Henry Hall Atrium 89

Expression of mutant horse heart cytochrome c peroxidase (HH CcP) was carried out using a recombinant bacterial expression system. Crystallization of HH CcP mutant E290K was realized with the formation of purified protein crystals. Although crystallization of this mutant was attained other mutant strains were found to be less receptive of the techniques used during the early stages of isolation. Crystal size was found to be diminutive and recrystallization for the sample will need to be performed to increase crystal dimensions for crystallography to be performed. Further development and honing of the procedures for cell cultures, protein isolation and purification was carried out through acclimatizing prior procedures to attain improved realizations of product though each stage of product collection.