Rescue of the Delorean Phenotype in Drosophila melanogaster

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology

Mentor Information

Georgette Sass

Department

Biology

Location

Kirkhof Center KC39

Start Date

11-4-2012 9:00 AM

Abstract

Drosophila melanogaster homozygous for the delorean mutation exhibit a phenotype with wings that are extended away from the body and noticeably curved downward. This is in contrast to the wings of wild-type flies that are held straight back over the body and not curved. The delorean phenotype is recessive and thought to be due to altered expression of the protein kinase N (pkn) gene during wing morphogenesis. Providing the PKN protein (i.e. the product of the wild-type pkn gene) to delorean flies should restore the wild-type phenotype if the delorean phenotype is due to a disruption of PKN gene expression. To rescue the mutant phenotype of the delorean flies we constructed a transformation vector using the pCasper-HS plasmid engineered to contain a cDNA sequence that could produce wild-type PKN. We generated an additional construct containing the wild-type pkn gene sequence in the transformation vector pUAST to activate PKN protein expression in specific tissues.

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Apr 11th, 9:00 AM

Rescue of the Delorean Phenotype in Drosophila melanogaster

Kirkhof Center KC39

Drosophila melanogaster homozygous for the delorean mutation exhibit a phenotype with wings that are extended away from the body and noticeably curved downward. This is in contrast to the wings of wild-type flies that are held straight back over the body and not curved. The delorean phenotype is recessive and thought to be due to altered expression of the protein kinase N (pkn) gene during wing morphogenesis. Providing the PKN protein (i.e. the product of the wild-type pkn gene) to delorean flies should restore the wild-type phenotype if the delorean phenotype is due to a disruption of PKN gene expression. To rescue the mutant phenotype of the delorean flies we constructed a transformation vector using the pCasper-HS plasmid engineered to contain a cDNA sequence that could produce wild-type PKN. We generated an additional construct containing the wild-type pkn gene sequence in the transformation vector pUAST to activate PKN protein expression in specific tissues.