Expression and Characterization of the Beta-Lactamase, ADC-7
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology
Mentor Information
Brad Wallar
Department
Chemistry
Location
Kirkhof Center KC12
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
²-Lactamases are bacterial enzymes that are responsible for much of the antibiotic resistance that exists in human health. While there are numerous ²-lactamases that have been identified and characterized. One relatively new ²-lactamase enzyme is ADC-7 (Acinetobacter-derived cephalosporinase-7); isolated from Acinetobacter bacteria that are emerging as opportunistic hospital pathogens demonstrating resistance to many antibiotic classes. In order to isolate and characterize ADC-7, we are using molecular biological techniques to generate a specific ADC-7 construct that will allow for the purification of large amounts of ADC-7 protein. This will provide enough ADC-7 for structural determination by X-ray crystallography. In addition, the pure ADC-7 enzyme is being subjected to kinetic assays to measure its ability to resist an array of antibiotics. Hopefully, our work to develop a structure/function relationship for ADC-7 will provide insight into bacterial antibiotic resistance.
Expression and Characterization of the Beta-Lactamase, ADC-7
Kirkhof Center KC12
²-Lactamases are bacterial enzymes that are responsible for much of the antibiotic resistance that exists in human health. While there are numerous ²-lactamases that have been identified and characterized. One relatively new ²-lactamase enzyme is ADC-7 (Acinetobacter-derived cephalosporinase-7); isolated from Acinetobacter bacteria that are emerging as opportunistic hospital pathogens demonstrating resistance to many antibiotic classes. In order to isolate and characterize ADC-7, we are using molecular biological techniques to generate a specific ADC-7 construct that will allow for the purification of large amounts of ADC-7 protein. This will provide enough ADC-7 for structural determination by X-ray crystallography. In addition, the pure ADC-7 enzyme is being subjected to kinetic assays to measure its ability to resist an array of antibiotics. Hopefully, our work to develop a structure/function relationship for ADC-7 will provide insight into bacterial antibiotic resistance.