Event Title
Creation of Salmonella Type 3 Secretion System Mutants
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
M. Aaron Baxter
Department
Biomedical Sciences
Location
Henry Hall Atrium 5
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
Salmonella enterica serovar Typhimurium is a bacteria which causes a self-limiting gastroenteritis in humans. Pathogenicity of Salmonella is dependent upon a series of genetic "islands" in the chromosome termed SPIs. SPI-1 contains the genes necessary to construct a type three secretion system (T3SS) that Salmonella uses to directly deliver virulence factors into targeted host intestinal cells. These virulence factors manipulate the host cell cytoskeleton to facilitate Salmonella entry into the cell. The T3SS is a needle-shaped structure composed of many proteins. The tip of this structure includes 2 proteins, SipD and PrgI. These proteins form the needle and trigger necessary for secretion. Working in collaboration with Bill Picking at Oklahoma State University, who is characterizing the function and assembly of the secretion needle, we are creating nonpolar prgI and sipD mutants within the Salmonella genome utilizing the method developed by Datsenko and Wanner.
Creation of Salmonella Type 3 Secretion System Mutants
Henry Hall Atrium 5
Salmonella enterica serovar Typhimurium is a bacteria which causes a self-limiting gastroenteritis in humans. Pathogenicity of Salmonella is dependent upon a series of genetic "islands" in the chromosome termed SPIs. SPI-1 contains the genes necessary to construct a type three secretion system (T3SS) that Salmonella uses to directly deliver virulence factors into targeted host intestinal cells. These virulence factors manipulate the host cell cytoskeleton to facilitate Salmonella entry into the cell. The T3SS is a needle-shaped structure composed of many proteins. The tip of this structure includes 2 proteins, SipD and PrgI. These proteins form the needle and trigger necessary for secretion. Working in collaboration with Bill Picking at Oklahoma State University, who is characterizing the function and assembly of the secretion needle, we are creating nonpolar prgI and sipD mutants within the Salmonella genome utilizing the method developed by Datsenko and Wanner.