Tools for Monitoring the Effects of Gene Manipulation on Cell Differentiation in the Embryonic Chick Midbrain

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences

Mentor Information

Merritt Taylor

Department

Biomedical Sciences

Location

Kirkhof Center KC58

Start Date

11-4-2012 9:00 AM

Keywords

Information, Innovation, and Technology, Health, Physical Science

Abstract

The chick embryonic midbrain offers an effective model to monitor the effects of gene overexpression or knockdown on neural progenitor differentiation at multiple different points during development. To better characterize the effect of gene manipulation, we are analyzing the expression pattern of midbrain progenitor domains in the normal embryonic chick mesencephalon using markers that have been established in the mouse. We also have developed a quantitative PCR approach to detect changes in midbrain progenitor marker expression in response to experimental manipulation of gene expression using in ovo electroporation. These approaches will allow investigators to readily screen for changes in progenitor differentiation and be able to better analyze the effect of genes on the differentiation of mesecephalic progenitors.

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Apr 11th, 9:00 AM

Tools for Monitoring the Effects of Gene Manipulation on Cell Differentiation in the Embryonic Chick Midbrain

Kirkhof Center KC58

The chick embryonic midbrain offers an effective model to monitor the effects of gene overexpression or knockdown on neural progenitor differentiation at multiple different points during development. To better characterize the effect of gene manipulation, we are analyzing the expression pattern of midbrain progenitor domains in the normal embryonic chick mesencephalon using markers that have been established in the mouse. We also have developed a quantitative PCR approach to detect changes in midbrain progenitor marker expression in response to experimental manipulation of gene expression using in ovo electroporation. These approaches will allow investigators to readily screen for changes in progenitor differentiation and be able to better analyze the effect of genes on the differentiation of mesecephalic progenitors.