The Role of CBL10 in Stamen Development in Arabidopsis thaliana
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology
Mentor Information
Margaret Dietrich
Department
Cell and Molecular Biology
Location
Henry Hall Atrium 1
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
Flowering plants are a primary source of food for humans. Therefore, it is of the utmost importance for us to understand plant flower development. Recently a gene encoding a calcineurin-B-like protein (CBL10) has been shown to play a role in flower development in A. thaliana. Mutations in this gene cause a breakdown in the stamen development pathway, resulting in nonfunctional stamens. Leaf and open flower cDNA was used to optimize PCR conditions for 8 of the genes in the pathway. Then RT-PCR was used to compare expression in open flowers of WT and cbl10 recovered (cbl10R) plants. Preliminary data show that 4 of the genes are expressed equally in these flowers, indicating that CBL10 acts in a subsequent step or that normal gene expression has been restored in recovered cbl10 flowers. In the future, gene expression will be studied in 3 stages of flower development: open flowers, opening flowers, and bud clusters, in 3 A. thaliana types: WT, cbl10 phenotype 2, and cbl10R.
The Role of CBL10 in Stamen Development in Arabidopsis thaliana
Henry Hall Atrium 1
Flowering plants are a primary source of food for humans. Therefore, it is of the utmost importance for us to understand plant flower development. Recently a gene encoding a calcineurin-B-like protein (CBL10) has been shown to play a role in flower development in A. thaliana. Mutations in this gene cause a breakdown in the stamen development pathway, resulting in nonfunctional stamens. Leaf and open flower cDNA was used to optimize PCR conditions for 8 of the genes in the pathway. Then RT-PCR was used to compare expression in open flowers of WT and cbl10 recovered (cbl10R) plants. Preliminary data show that 4 of the genes are expressed equally in these flowers, indicating that CBL10 acts in a subsequent step or that normal gene expression has been restored in recovered cbl10 flowers. In the future, gene expression will be studied in 3 stages of flower development: open flowers, opening flowers, and bud clusters, in 3 A. thaliana types: WT, cbl10 phenotype 2, and cbl10R.