Influence of Glucose and Serum on Connexin 43 Expression in Cultured Mouse Endothelial Cells

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences, Chemistry

Mentor Information

David Kurjiaka

Department

Biomedical Sciences

Location

Kirkhof Center KC9

Start Date

10-4-2013 10:00 AM

End Date

10-4-2013 11:00 AM

Keywords

Physical Science

Abstract

The supplier (ATCC) of cultured mouse endothelial cells (bEnd.3) states that those cells should be maintained in a high glucose (10% serum) environment. Previous data gathered from our lab demonstrated that media composition (glucose and serum) impacts the growth rate of these cells. As connexin expression alters cell growth, we were interested in determining whether glucose and serum might alter connexin expression in a way that would affect cell growth. Others have shown that glucose increases endothelial cell connexin 43 expression in vivo. Mouse endothelial cells were grown in mixtures of high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with either 10% or 1% serum (fetal bovine FBS). We expect that high glucose and 10% serum, will increase connexin 43 expression which could contribute to the increased growth rate.

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Apr 10th, 10:00 AM Apr 10th, 11:00 AM

Influence of Glucose and Serum on Connexin 43 Expression in Cultured Mouse Endothelial Cells

Kirkhof Center KC9

The supplier (ATCC) of cultured mouse endothelial cells (bEnd.3) states that those cells should be maintained in a high glucose (10% serum) environment. Previous data gathered from our lab demonstrated that media composition (glucose and serum) impacts the growth rate of these cells. As connexin expression alters cell growth, we were interested in determining whether glucose and serum might alter connexin expression in a way that would affect cell growth. Others have shown that glucose increases endothelial cell connexin 43 expression in vivo. Mouse endothelial cells were grown in mixtures of high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with either 10% or 1% serum (fetal bovine FBS). We expect that high glucose and 10% serum, will increase connexin 43 expression which could contribute to the increased growth rate.