Event Title

A Look Into the Past: Photo-Physiology of Cyanobacterial Mat Pigments in Alpena, Michigan and Lake Huron Sinkholes

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences

Mentor Information

Bopi Biddanda

Department

Annis Water Resource Institute (AWRI)

Location

Henry Hall Atrium 95

Start Date

10-4-2013 12:00 PM

End Date

10-4-2013 1:00 PM

Keywords

Life Science

Abstract

Cyanobacteria were key to oxygenating the atmosphere 2.5 billion years ago (bya). My study examined light harvesting pigments and their response to different light conditions in modern purple cyanobacterial mats that reside in the bottom of the deep, low-light Middle Island sinkhole and the shallow high-light El Cajon springs. Chlorophyll a, phycocyanin (PC) and phycoerythrin (PE) were extracted for analysis and pigment ratios were compared between locations. Photosynthetic yield was taken using a Dive-PAM to investigate the photo-physiology of the mats, on location and in the lab. Pigment concentrations from the high-light mats showed decreased pigment concentrations as light intensity increased, while low-light mat pigment concentrations increased with increasing light intensity. Further, PE:PC showed that El Cajon cyanobacteria express more PC, while Middle Island mat express more PE suggesting these modern cyanobacteria actively vary their pigment composition in response to available light.

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Apr 10th, 12:00 PM Apr 10th, 1:00 PM

A Look Into the Past: Photo-Physiology of Cyanobacterial Mat Pigments in Alpena, Michigan and Lake Huron Sinkholes

Henry Hall Atrium 95

Cyanobacteria were key to oxygenating the atmosphere 2.5 billion years ago (bya). My study examined light harvesting pigments and their response to different light conditions in modern purple cyanobacterial mats that reside in the bottom of the deep, low-light Middle Island sinkhole and the shallow high-light El Cajon springs. Chlorophyll a, phycocyanin (PC) and phycoerythrin (PE) were extracted for analysis and pigment ratios were compared between locations. Photosynthetic yield was taken using a Dive-PAM to investigate the photo-physiology of the mats, on location and in the lab. Pigment concentrations from the high-light mats showed decreased pigment concentrations as light intensity increased, while low-light mat pigment concentrations increased with increasing light intensity. Further, PE:PC showed that El Cajon cyanobacteria express more PC, while Middle Island mat express more PE suggesting these modern cyanobacteria actively vary their pigment composition in response to available light.