Event Title
Investigating the Potential of Cellular Focusing and Separation by Capillary Isotachophoresis
Presentation Type
Poster/Portfolio
Presenter Major(s)
Chemistry
Mentor Information
Andrew Lantz
Department
Chemistry
Location
Henry Hall Atrium 34
Start Date
10-4-2013 3:00 PM
End Date
10-4-2013 4:00 PM
Keywords
Life Science, Physical Science
Abstract
Capillary isotachophoresis has long been a useful technique for small molecule separations. This research serves to investigate its potential in the separation of whole cell mixtures. The behaviors of bacterial and fungal species in standard capillary zone electrophoresis are compared to those in isotachophoresis in order to make qualitative assessments. Electrophoretic mobility ranges of different cell species were cataloged and bracketed with ions of higher and lower mobility in isotachphoresis. It is likely that the focusing abilities of isotachophoresis will be able to overcome band broadening caused by heterogeneity in cell cultures and the formation of cellular aggregates. The collected data suggests isotachophoresis may be potentially used in standardized testing of unknown cell cultures.
Investigating the Potential of Cellular Focusing and Separation by Capillary Isotachophoresis
Henry Hall Atrium 34
Capillary isotachophoresis has long been a useful technique for small molecule separations. This research serves to investigate its potential in the separation of whole cell mixtures. The behaviors of bacterial and fungal species in standard capillary zone electrophoresis are compared to those in isotachophoresis in order to make qualitative assessments. Electrophoretic mobility ranges of different cell species were cataloged and bracketed with ions of higher and lower mobility in isotachphoresis. It is likely that the focusing abilities of isotachophoresis will be able to overcome band broadening caused by heterogeneity in cell cultures and the formation of cellular aggregates. The collected data suggests isotachophoresis may be potentially used in standardized testing of unknown cell cultures.