Date Approved

8-2023

Graduate Degree Type

Thesis

Degree Name

Biomedical Sciences (M.H.S.)

Degree Program

Biomedical Sciences

First Advisor

Merritt Delano-Taylor

Second Advisor

John Capodilupo

Third Advisor

Eric Ramsson

Fourth Advisor

Daniel Bergman

Fifth Advisor

Christopher Pearl

Academic Year

2022/2023

Abstract

This thesis presents significant findings regarding the role of PM-Nato3 in its interaction with developing neurons in the context of Parkinson's disease (PD) and regenerative medicine. We investigated the effects of PM-Nato3 on dopamine (DA) neurogenesis under different culture conditions, both in vitro and in vivo. In the standard dopaminergic culture condition, PM-Nato3 potentially increased the speed of DA neuron production but did not significantly increase the yield of DA neurons. In a minimal culture condition, there was no notable difference between the control and PM-Nato3 conditions, suggesting minimal impact on DA neurogenesis. In vivo studies using a mouse model revealed that PM-Nato3 did not elevate neuroprotective transcription factors (Foxa2, En1, Nurr1) but showed a modest increase in TH mRNA expression, indicating potential effects on dopaminergic neurons. These findings provide insights into the complex relationship between PM- Nato3 and neuronal development. Further research is needed to understand the underlying molecular mechanisms and signaling pathways involved. This knowledge could pave the way for more effective treatments and regenerative strategies for PD, establishing a foundation for future investigations into PM-Nato3, contributing to advancements in Parkinson's disease research and regenerative medicine.

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