Identifying an Atypical Actin Binding Domain in the Fission Yeast Mid1 Scaffold

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology, Chemistry

Mentor Information

Dawn Clifford Hart, hartdaw@gvsu.edu

Department

Cell and Molecular Biology

Location

Henry Hall Atrium 75

Start Date

13-4-2011 1:00 PM

End Date

13-4-2011 2:00 PM

Keywords

Health, Illness, and Healing, Life Science

Abstract

The scaffolding protein Mid1, found in the fission yeast S. pombe, functions in the assembly and placement of the actin contractile ring, which is required for cell division. The placement and functionality of the division septum corresponds to the placement of the ring. Therefore, identifying contractile ring proteins that directly associate with Mid1 will contribute to our understanding of proper cell division and equal transfer of the cellular contents. Our preliminary results suggest that Mid1 contributes to the formation and placement of the actin contractile ring through direct association with F-Actin filaments. The main goals of this research project are to identify the actin binding domain in Mid1 and analyze the consequence of disrupting the interaction. To test this, we are using actin cosedimentation assays. After identifying the actin binding region within Mid1, mutants with alterations to the actin binding region will be generated and analyzed for cell division defects.

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Apr 13th, 1:00 PM Apr 13th, 2:00 PM

Identifying an Atypical Actin Binding Domain in the Fission Yeast Mid1 Scaffold

Henry Hall Atrium 75

The scaffolding protein Mid1, found in the fission yeast S. pombe, functions in the assembly and placement of the actin contractile ring, which is required for cell division. The placement and functionality of the division septum corresponds to the placement of the ring. Therefore, identifying contractile ring proteins that directly associate with Mid1 will contribute to our understanding of proper cell division and equal transfer of the cellular contents. Our preliminary results suggest that Mid1 contributes to the formation and placement of the actin contractile ring through direct association with F-Actin filaments. The main goals of this research project are to identify the actin binding domain in Mid1 and analyze the consequence of disrupting the interaction. To test this, we are using actin cosedimentation assays. After identifying the actin binding region within Mid1, mutants with alterations to the actin binding region will be generated and analyzed for cell division defects.