Event Title
Impact of Hypergravity Exposure on the Mammary Gland Cytoskeletal Organization in the Rat
Presentation Type
Poster/Portfolio
Presenter Major(s)
Cell and Molecular Biology
Mentor Information
Osman Patel, patelo@gvsu.edu
Department
Cell and Molecular Biology
Location
Kirkhof Center KC25
Start Date
13-4-2011 9:00 AM
End Date
13-4-2011 10:00 AM
Keywords
Environment, Health and Wellness, Life Science, Technology
Abstract
Differential and exponential growth of the mammary gland occurs during pregnancy under hormonal influences. This dynamic epithelial differentiation is dependent on an integral cytoskeletal support structure. Chronic exposure of pregnant rats to hypergravity (HG) diminishes postpartum mammary gland “output†that is independent of prolactin and glucocorticoid secretion. Therefore, the objective of this study is to determine the effects of HG exposure from mid- to late pregnancy on pre-partum distribution of the cytoskeletal components in the mammary gland. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and formalin-fixed. Immunolocalization studies of alpha-smooth muscle actin and tubulin using specific antibodies directed against these proteins in HG exposed and control rats are completed. Data from structure and component analysis will be presented.
Impact of Hypergravity Exposure on the Mammary Gland Cytoskeletal Organization in the Rat
Kirkhof Center KC25
Differential and exponential growth of the mammary gland occurs during pregnancy under hormonal influences. This dynamic epithelial differentiation is dependent on an integral cytoskeletal support structure. Chronic exposure of pregnant rats to hypergravity (HG) diminishes postpartum mammary gland “output†that is independent of prolactin and glucocorticoid secretion. Therefore, the objective of this study is to determine the effects of HG exposure from mid- to late pregnancy on pre-partum distribution of the cytoskeletal components in the mammary gland. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and formalin-fixed. Immunolocalization studies of alpha-smooth muscle actin and tubulin using specific antibodies directed against these proteins in HG exposed and control rats are completed. Data from structure and component analysis will be presented.