Impact of Hypergravity Exposure on the Mammary Gland Cytoskeletal Organization in the Rat

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology

Mentor Information

Osman Patel, patelo@gvsu.edu

Department

Cell and Molecular Biology

Location

Kirkhof Center KC25

Start Date

13-4-2011 9:00 AM

End Date

13-4-2011 10:00 AM

Keywords

Environment, Health and Wellness, Life Science, Technology

Abstract

Differential and exponential growth of the mammary gland occurs during pregnancy under hormonal influences. This dynamic epithelial differentiation is dependent on an integral cytoskeletal support structure. Chronic exposure of pregnant rats to hypergravity (HG) diminishes postpartum mammary gland “output” that is independent of prolactin and glucocorticoid secretion. Therefore, the objective of this study is to determine the effects of HG exposure from mid- to late pregnancy on pre-partum distribution of the cytoskeletal components in the mammary gland. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and formalin-fixed. Immunolocalization studies of alpha-smooth muscle actin and tubulin using specific antibodies directed against these proteins in HG exposed and control rats are completed. Data from structure and component analysis will be presented.

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Apr 13th, 9:00 AM Apr 13th, 10:00 AM

Impact of Hypergravity Exposure on the Mammary Gland Cytoskeletal Organization in the Rat

Kirkhof Center KC25

Differential and exponential growth of the mammary gland occurs during pregnancy under hormonal influences. This dynamic epithelial differentiation is dependent on an integral cytoskeletal support structure. Chronic exposure of pregnant rats to hypergravity (HG) diminishes postpartum mammary gland “output” that is independent of prolactin and glucocorticoid secretion. Therefore, the objective of this study is to determine the effects of HG exposure from mid- to late pregnancy on pre-partum distribution of the cytoskeletal components in the mammary gland. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and formalin-fixed. Immunolocalization studies of alpha-smooth muscle actin and tubulin using specific antibodies directed against these proteins in HG exposed and control rats are completed. Data from structure and component analysis will be presented.