Impact of Hypergravity Exposure on Pregnant Rat Mammary Lobular Dimensions

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology

Mentor Information

Osman Patel, patelo@gvsu.edu

Department

Cell and Molecular Biology

Location

Kirkhof Center KC40

Start Date

13-4-2011 9:00 AM

End Date

13-4-2011 10:00 AM

Keywords

Environment, Health and Wellness, Life Science, Technology

Abstract

Mammary gland output is determined by the number of secretory epithelial cells. Moreover, growth and differentiation of the mammary gland predominately occurs during pregnancy. Previous studies show that exposure to hypergravity (HG) during pregnancy impacts metabolic activity of the dam during lactation. Therefore, the objective of this study is to evaluate the effects of hypergravity exposure from mid- to late pregnancy on pre-partum dimensions of the mammary gland lobules. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and processed for immunohistochemistry.Tissue was formalin fixed and paraffin embedded for histological and immunohistochemical characterization of cellular structures using antibody directed staining and image analysis of structure, area and component analysis.Immunolocalization studies of HG exposed and control rats are completed and quantification data will be presented.

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Apr 13th, 9:00 AM Apr 13th, 10:00 AM

Impact of Hypergravity Exposure on Pregnant Rat Mammary Lobular Dimensions

Kirkhof Center KC40

Mammary gland output is determined by the number of secretory epithelial cells. Moreover, growth and differentiation of the mammary gland predominately occurs during pregnancy. Previous studies show that exposure to hypergravity (HG) during pregnancy impacts metabolic activity of the dam during lactation. Therefore, the objective of this study is to evaluate the effects of hypergravity exposure from mid- to late pregnancy on pre-partum dimensions of the mammary gland lobules. Pregnant rats were exposed to either 2g (HG) or 1g (control) from days 11 to 20 of gestation (G20). On G20, mammary tissue was collected and processed for immunohistochemistry.Tissue was formalin fixed and paraffin embedded for histological and immunohistochemical characterization of cellular structures using antibody directed staining and image analysis of structure, area and component analysis.Immunolocalization studies of HG exposed and control rats are completed and quantification data will be presented.