Isotachophoretic Focusing of Bacteria and Fungi for Analysis

Presentation Type

Poster/Portfolio

Presenter Major(s)

Chemistry, Russian Studies

Mentor Information

Andrew Lantz

Department

Chemistry

Location

Henry Hall Atrium 42

Start Date

11-4-2012 9:00 AM

Keywords

Life Science, Physical Science

Abstract

In this experiment we attempt to use the isotachophoresis mode of capillary electrophoresis to focus and potentially separate bacterial and fungal species. Isotachophoresis is typically used in biochemistry to concentrate and separate protein samples based on their electrophoretic mobilities as compared to the other components in a multi-electrolyte system. The same basic principle may be applied to the analysis of whole cells by exploiting their differences in size and surface charges. Different bacteria such as gram positive and gram negative have different surface characteristics as well as being vastly different from that of fungi. By focusing the cells into distinct zones based on these surface characteristics it would be possible to differentiate between species and thus have a fast and inexpensive test to determine the cause of sample contamination.

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Apr 11th, 9:00 AM

Isotachophoretic Focusing of Bacteria and Fungi for Analysis

Henry Hall Atrium 42

In this experiment we attempt to use the isotachophoresis mode of capillary electrophoresis to focus and potentially separate bacterial and fungal species. Isotachophoresis is typically used in biochemistry to concentrate and separate protein samples based on their electrophoretic mobilities as compared to the other components in a multi-electrolyte system. The same basic principle may be applied to the analysis of whole cells by exploiting their differences in size and surface charges. Different bacteria such as gram positive and gram negative have different surface characteristics as well as being vastly different from that of fungi. By focusing the cells into distinct zones based on these surface characteristics it would be possible to differentiate between species and thus have a fast and inexpensive test to determine the cause of sample contamination.