Initial Cell Size Affects the Hormone Response in Physcomitrella patens

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology

Mentor Information

Margaret Dietrich

Department

Cell and Molecular Biology

Location

Henry Hall Atrium 91

Start Date

11-4-2012 9:00 AM

Keywords

Life Science

Abstract

How cells perceive and respond to hormones is largely unknown, although their role in development is crucial. When initial cells of the moss, Physcomitrella patens, are exposed to the plant hormone cytokinin, normal tip growth transitions into the development of a leafy gametophyte. In order to study this signaling process, a mutant was identified which produces initial cells unable to respond to cytokinin. These initial cells appear to grow much faster than those of the wild type, which potentially removes the window of competency for response to the hormone. TAIL PCR was used to determine the sequence upstream of the insertion site and identify the locus. The locus does not appear to contain coding sequence, but does include retrotransposon sequence and 10bp tandem repeats, which precludes reconstruction of the mutant. Instead, formation of initial cells will be monitored to characterize the mutant in an effort to understand what confers competency for the cytokinin response.

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Apr 11th, 9:00 AM

Initial Cell Size Affects the Hormone Response in Physcomitrella patens

Henry Hall Atrium 91

How cells perceive and respond to hormones is largely unknown, although their role in development is crucial. When initial cells of the moss, Physcomitrella patens, are exposed to the plant hormone cytokinin, normal tip growth transitions into the development of a leafy gametophyte. In order to study this signaling process, a mutant was identified which produces initial cells unable to respond to cytokinin. These initial cells appear to grow much faster than those of the wild type, which potentially removes the window of competency for response to the hormone. TAIL PCR was used to determine the sequence upstream of the insertion site and identify the locus. The locus does not appear to contain coding sequence, but does include retrotransposon sequence and 10bp tandem repeats, which precludes reconstruction of the mutant. Instead, formation of initial cells will be monitored to characterize the mutant in an effort to understand what confers competency for the cytokinin response.