Influence of Glucose on Growth of Cultured Mouse Endothelial Cells

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences, Chemistry, Biopsychology, Physician Assistant Studies, Clinical Laboratory Science

Mentor Information

David Kurjiaka

Department

Biomedical Sciences

Location

Henry Hall Atrium 50

Start Date

11-4-2012 9:00 AM

Keywords

Life Science

Abstract

The supplier (ATCC) of cultured mouse endothelial cells states that those cells should be maintained in a high glucose environment. As these are the only cultured endothelial cells available commercially that do not require growth supplements in addition to the FBS, we were interested in whether the media glucose concentration influenced the rates of cell division (growth) in these cells. The growth of mouse endothelial cell (bEnd.3) division was assessed in high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with 10% FBS-DMEM. The rate of endothelial cell growth was reduced when glucose was closer to physiological concentrations (5.56 mM/L = 100 mg/dl). When the FBS was lowered/removed from the media, the cells continued to grow (although more slowly than in 10% FBS). As the cells in 5.6 mM grew even slower, high glucose appears to stimulate the release of growth factors from the endothelial cells that are necessary for their growth.

This document is currently not available here.

Share

COinS
 
Apr 11th, 9:00 AM

Influence of Glucose on Growth of Cultured Mouse Endothelial Cells

Henry Hall Atrium 50

The supplier (ATCC) of cultured mouse endothelial cells states that those cells should be maintained in a high glucose environment. As these are the only cultured endothelial cells available commercially that do not require growth supplements in addition to the FBS, we were interested in whether the media glucose concentration influenced the rates of cell division (growth) in these cells. The growth of mouse endothelial cell (bEnd.3) division was assessed in high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with 10% FBS-DMEM. The rate of endothelial cell growth was reduced when glucose was closer to physiological concentrations (5.56 mM/L = 100 mg/dl). When the FBS was lowered/removed from the media, the cells continued to grow (although more slowly than in 10% FBS). As the cells in 5.6 mM grew even slower, high glucose appears to stimulate the release of growth factors from the endothelial cells that are necessary for their growth.