Event Title
Influence of Glucose on Growth of Cultured Mouse Endothelial Cells
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences, Chemistry, Biopsychology, Physician Assistant Studies, Clinical Laboratory Science
Mentor Information
David Kurjiaka
Department
Biomedical Sciences
Location
Henry Hall Atrium 50
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
The supplier (ATCC) of cultured mouse endothelial cells states that those cells should be maintained in a high glucose environment. As these are the only cultured endothelial cells available commercially that do not require growth supplements in addition to the FBS, we were interested in whether the media glucose concentration influenced the rates of cell division (growth) in these cells. The growth of mouse endothelial cell (bEnd.3) division was assessed in high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with 10% FBS-DMEM. The rate of endothelial cell growth was reduced when glucose was closer to physiological concentrations (5.56 mM/L = 100 mg/dl). When the FBS was lowered/removed from the media, the cells continued to grow (although more slowly than in 10% FBS). As the cells in 5.6 mM grew even slower, high glucose appears to stimulate the release of growth factors from the endothelial cells that are necessary for their growth.
Influence of Glucose on Growth of Cultured Mouse Endothelial Cells
Henry Hall Atrium 50
The supplier (ATCC) of cultured mouse endothelial cells states that those cells should be maintained in a high glucose environment. As these are the only cultured endothelial cells available commercially that do not require growth supplements in addition to the FBS, we were interested in whether the media glucose concentration influenced the rates of cell division (growth) in these cells. The growth of mouse endothelial cell (bEnd.3) division was assessed in high (4.5 g/L = 25 mM/L) and low (1 g/L = 5.6 mM/L) glucose with 10% FBS-DMEM. The rate of endothelial cell growth was reduced when glucose was closer to physiological concentrations (5.56 mM/L = 100 mg/dl). When the FBS was lowered/removed from the media, the cells continued to grow (although more slowly than in 10% FBS). As the cells in 5.6 mM grew even slower, high glucose appears to stimulate the release of growth factors from the endothelial cells that are necessary for their growth.