Characterization of Lethal Deficiencies in the Gi-alpha 65A Gene in Drosophila melanogaster
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biomedical Sciences
Mentor Information
Martin Burg
Department
Biomedical Sciences
Location
Henry Hall Atrium 78
Start Date
11-4-2012 9:00 AM
Keywords
Life Science
Abstract
P-element transposition is useful for generating new deletion mutations in D. melanogaster to increase our knowledge of specific genes and their roles in a variety of biological processes, including neurogenesis. Specific pHdc-5'UTR-eGFP transgene combinations had an effect on the histaminergic nervous system by eliminating histaminergic neurons in the central nervous system of D. melanogaster. One of these transgenes was inserted into the Gi±65A gene, which has been implicated in the process of neural differentiation. Flies containing the pHdc-5UTR-eGFP transgene in Gi±65A were exposed to the transposase source 2-3 to generate deletion events. Lethal excisions from of the Gi±65A insert needed to be examined further for chromosomal abnormalities. Molecular analysis of the deletions that do not have obvious chromosomal aberrations will be carried out to determine where the deletion in the gene may be, and if there is an effect on the development of the histaminergic nervous system.
Characterization of Lethal Deficiencies in the Gi-alpha 65A Gene in Drosophila melanogaster
Henry Hall Atrium 78
P-element transposition is useful for generating new deletion mutations in D. melanogaster to increase our knowledge of specific genes and their roles in a variety of biological processes, including neurogenesis. Specific pHdc-5'UTR-eGFP transgene combinations had an effect on the histaminergic nervous system by eliminating histaminergic neurons in the central nervous system of D. melanogaster. One of these transgenes was inserted into the Gi±65A gene, which has been implicated in the process of neural differentiation. Flies containing the pHdc-5UTR-eGFP transgene in Gi±65A were exposed to the transposase source 2-3 to generate deletion events. Lethal excisions from of the Gi±65A insert needed to be examined further for chromosomal abnormalities. Molecular analysis of the deletions that do not have obvious chromosomal aberrations will be carried out to determine where the deletion in the gene may be, and if there is an effect on the development of the histaminergic nervous system.