Characterization of PkndlnΔ5, a Derivative Allele of the Delorean Mutation Associated with the Protein Kinase N Gene in Drosophila melanogaster
Presentation Type
Poster/Portfolio
Presenter Major(s)
Biology
Mentor Information
Georgette Sass
Department
Biology
Location
Kirkhof Center KC54
Start Date
11-4-2012 9:00 AM
Abstract
Wings of Drosophila melanogaster that are homozygous for the delorean mutation (Pkndln) are held out and arched downward in contrast to the closely held, flat wings of the wild type. The delorean mutation is the result of a P-element that inserted into the first intron of the Protein kinase N (Pkn) gene. To understand the molecular basis of the delorean phenotype we have generated a deletion derivative of the Pkndln allele, PkndlnΔ5. Flies that are homozygous for this derivative show distinct differences in phenotype severity from Pkndln; the wings are less severe whereas fertility defects are more severe. In addition, PkndlnΔ5demonstrates that the delorean phenotype is transvection-dependent. The molecular lesion associated with the PkndlnΔ5allele is the loss of mini-white+ gene sequence from within the associated P-element. We present our analysis of the PkndlnΔ5derivative with respect to the role of the Pkn gene in wing morphogenesis as well as its prospective role in oogenesis.
Characterization of PkndlnΔ5, a Derivative Allele of the Delorean Mutation Associated with the Protein Kinase N Gene in Drosophila melanogaster
Kirkhof Center KC54
Wings of Drosophila melanogaster that are homozygous for the delorean mutation (Pkndln) are held out and arched downward in contrast to the closely held, flat wings of the wild type. The delorean mutation is the result of a P-element that inserted into the first intron of the Protein kinase N (Pkn) gene. To understand the molecular basis of the delorean phenotype we have generated a deletion derivative of the Pkndln allele, PkndlnΔ5. Flies that are homozygous for this derivative show distinct differences in phenotype severity from Pkndln; the wings are less severe whereas fertility defects are more severe. In addition, PkndlnΔ5demonstrates that the delorean phenotype is transvection-dependent. The molecular lesion associated with the PkndlnΔ5allele is the loss of mini-white+ gene sequence from within the associated P-element. We present our analysis of the PkndlnΔ5derivative with respect to the role of the Pkn gene in wing morphogenesis as well as its prospective role in oogenesis.