Changes in Vascular Reactivity of Mesenteric Porcine Arteries Following Hyperbaric Treatment

Presentation Type

Poster/Portfolio

Presenter Major(s)

Biomedical Sciences

Mentor Information

Francis Sylvester

Department

Biomedical Sciences

Location

Kirkhof Center KC7

Start Date

11-4-2012 9:00 AM

Keywords

Health

Abstract

The objective of this study was to evaluate the changes in vascular reactivity of mesenteric arteries resulting from hyperbaric oxygen treatment. It was hypothesized that hyperbaric oxygen treatment differentially alters vascular reactivity in mesenteric arteries. Arteries from porcine mesentery were dissected and then subjected to a 2-hour hyperbaric treatment in 100% oxygen, 100% nitrogen, or room air at 1.75 ATA. Following a 1-hour equilibration in Krebs-Henseleit solution arteries were treated with potassium chloride to assess viability. Subsequently, arteries were treated with increasing concentrations of phenylephrine and then sodium nitroprusside to measure changes in vascular reactivity. Treatment with hyperbaric oxygen augmented vascular responses to phenylephrine and sodium nitroprusside when compared to arteries treated with hyperbaric nitrogen. The results from these studies provide basic insight into the physiological effects of hyperbaric oxygen treatment.

This document is currently not available here.

Share

COinS
 
Apr 11th, 9:00 AM

Changes in Vascular Reactivity of Mesenteric Porcine Arteries Following Hyperbaric Treatment

Kirkhof Center KC7

The objective of this study was to evaluate the changes in vascular reactivity of mesenteric arteries resulting from hyperbaric oxygen treatment. It was hypothesized that hyperbaric oxygen treatment differentially alters vascular reactivity in mesenteric arteries. Arteries from porcine mesentery were dissected and then subjected to a 2-hour hyperbaric treatment in 100% oxygen, 100% nitrogen, or room air at 1.75 ATA. Following a 1-hour equilibration in Krebs-Henseleit solution arteries were treated with potassium chloride to assess viability. Subsequently, arteries were treated with increasing concentrations of phenylephrine and then sodium nitroprusside to measure changes in vascular reactivity. Treatment with hyperbaric oxygen augmented vascular responses to phenylephrine and sodium nitroprusside when compared to arteries treated with hyperbaric nitrogen. The results from these studies provide basic insight into the physiological effects of hyperbaric oxygen treatment.