The Maternal Effect Phenotype of Delorean in Drosophila melanogaster

Presentation Type

Poster/Portfolio

Presenter Major(s)

Cell and Molecular Biology

Mentor Information

Georgette Sass

Department

Biology

Location

Henry Hall Atrium 82

Start Date

10-4-2013 10:00 AM

End Date

10-4-2013 11:00 AM

Keywords

Life Science

Abstract

The delorean allele is a gain-of-function mutation in Drosophila melanogaster that is caused by a transposon insertion in the first intron of the Protein kinase N (pkn) gene. It is known that the pkn gene in Drosophila melanogaster is required in embryonic development during the process of dorsal closure, establishing this kinase as a requirement for developing embryos. The dorsal closure phenotype is rescued when the normal PKN protein is supplied to mutant flies. Here, we study the role of pkn in oogenesis. Using the Dominant Female Sterile Flipase Recombinant Technique (DFS-FRT) germline, homozygous delorean clones were generated to quantify the maternal-effect phenotype. We establish pkn as a maternal-effect phenotype where females exhibit effective sterility due to a significant reduction in egg production. The eggs produced by delorean germline clones are either not fertilizable or if fertilized, generate embryos that fail to complete embryogenesis.

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Apr 10th, 10:00 AM Apr 10th, 11:00 AM

The Maternal Effect Phenotype of Delorean in Drosophila melanogaster

Henry Hall Atrium 82

The delorean allele is a gain-of-function mutation in Drosophila melanogaster that is caused by a transposon insertion in the first intron of the Protein kinase N (pkn) gene. It is known that the pkn gene in Drosophila melanogaster is required in embryonic development during the process of dorsal closure, establishing this kinase as a requirement for developing embryos. The dorsal closure phenotype is rescued when the normal PKN protein is supplied to mutant flies. Here, we study the role of pkn in oogenesis. Using the Dominant Female Sterile Flipase Recombinant Technique (DFS-FRT) germline, homozygous delorean clones were generated to quantify the maternal-effect phenotype. We establish pkn as a maternal-effect phenotype where females exhibit effective sterility due to a significant reduction in egg production. The eggs produced by delorean germline clones are either not fertilizable or if fertilized, generate embryos that fail to complete embryogenesis.